Coding
Part:BBa_K320001:Design
Designed by: Perrudet Christian Group: iGEM10_EPF_Lausanne (2010-10-18)
Immunotoxin against P.Berghei
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 116
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 28
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 957 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 472
Illegal BsaI site found at 699
Design Notes
Sequence was optimized for expression in E. coli.
Source
Coding sequence comes from [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X [1]].