Generator

Part:BBa_K395702:Design

Designed by: Yumiko Kinoshita   Group: iGEM10_Tokyo_Tech   (2010-10-02)
Revision as of 12:49, 5 October 2010 by Kino (Talk | contribs) (→‎Design Notes)

rbs+crtZ under pBad promoter for double plasmids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

・This operon is under arabinose inducible promoter.

・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.

Source

Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.

This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158.

References