Generator
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Part:BBa_K314203:Design
Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger Group: iGEM10_Washington (2010-10-19)
HSL inducible toxin antitoxin, Tse2 + Tsi2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 1340
Illegal NgoMIV site found at 1465
Illegal NgoMIV site found at 1795 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Illegal SapI.rc site found at 1790
Design Notes
This construct was originally tested in Psb3k3 with Bglbrick prefixes and suffixes
Source
Pseudomonas aeruginosa
References
Cell Host Microbe. 2010 Jan 21;7(1):25-37