Generator
Part:BBa_K358006:Design
Designed by: Ken Kajita Group: iGEM10_Kyoto (2010-09-19)
lambda lysis cassette with terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed PCR to get the lysis cassette[SRRz] and inserted before double terminator[BBa_B0015].
As the template, we used λ -Hind Ⅲ digest[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].
Forward primer:
Reverse primer:
Source
Used λ -Hind digest and BBa_B0015.