Reporter

Part:BBa_K389401:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)
Revision as of 14:20, 11 October 2010 by Registry (Talk | contribs)

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Sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 2639
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 818
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2545


Design Notes

arabinose promoter removed, GFP exchanged with luciferase compared to the original part BBa_I746370


Source

  • luciferase from pGL4.10luc2 (Promega)
  • the rest from the parts.igem

References