Protein_Domain
mCherry

Part:BBa_J18932

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)
Revision as of 18:54, 26 January 2010 by Raik (Talk | contribs)

mCherry RFP

Red fluorescent protein derived from DsRed.

Advantages:

  • fast folding and maturation
  • bright and photo-stable

Degradation issues:

  • Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
  • SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter
//proteindomain
Parameters
None