Translational_Unit

Part:BBa_K329000

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-12)
Revision as of 16:28, 12 October 2010 by Rpantier (Talk | contribs)

Medium RBS (B0032) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Medium RBS upstream (BBa_B0032). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see BBa_K329003, BBa_K329004))

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 703
    Illegal NgoMIV site found at 863
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None