Coding
virA

Part:BBa_K389001

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 10:00, 8 October 2010 by Nkessler (Talk | contribs) (→‎Usage and Biology)

VirA receptor

The VirA receptor is used by Agrobacterium tumefaciens to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates vir promoters. These promoters control genes which are used for infecting the injured plant.

This virA gene was isolated from the TI-plasmid of A. tumefaciens C58. An illegal PstI restriction site was removed by site-directed mutagenesis.


Usage and Biology

The VirA receptor of Agrobacterium tumefaciens

For a working VirA/G-receptor system, you also need the virG BioBrick BBa_K389002 and a vir promoter BBa_K389003. You can find an already assembled VirA/G-receptor system here: BBa_K389017.

This receptor recognizes acetosyringone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 586
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1707


[edit]
Categories
//cds/membrane/receptor
//cds/receptor
//chassis/prokaryote/ecoli
Parameters
None