Coding

Part:BBa_K322237:Design

Designed by: Chris French and William Galdric Rostain   Group: iGEM10_Edinburgh   (2010-09-26)
Revision as of 10:55, 26 September 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Firefly luciferase from Photinus pyralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

Forward sequence looks good. Reverse sequence missing the base between the stop codon and SpeI site. This is also missing in the S284T clone (see below) suggesting that it is missing in the primer, or did we use a vector based primer? In which case it would suggest that it was missing in the original clone. Trivial but annoying.



Source

The origional sequence is from Photinus pyralis, a the gene here is derived from BioBrick BBa-I712019.

References