Composite

Part:BBa_K322140:Design

Designed by: Richard Partridge-Hicks and Maria Kowal   Group: iGEM10_Edinburgh   (2010-10-07)
Revision as of 14:59, 7 October 2010 by Registry (Talk | contribs)

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lac promoter + luxAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1657


Design Notes

J33207 Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.

K322139 Notes: we prepared this for two reasons: firstly to resolve an issue with the SpeI site in our submitted version of K216008 (for some reason, every clone we sequenced seemed to have an error, not always the same error, in the suffix SpeI site), and to put it in pSB1C3, since this seems to be the new standard vector for the Registry.



Source

J33207 The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct. References

K322139 Source: genomic DNA of Xenorhabdus (Photorhabdus) luminescens Hb (actually, we used our BioBrick from last year, BBa_K216016 as template).

References