Composite

Part:BBa_K389014:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-28)
Revision as of 12:51, 7 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

VirA/G screening system test


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


Design Notes

  • The virA gene is under the control of a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
  • double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low
  • only for testing the screening principle of mutated virA screening

Source

A. tumefaciens C58 TI-plasmid, parts.igem, Mr. Gene

References