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Sample It's complicated

Experience: Works

Composite

Part:BBa_K389012:Design

Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-09-11)

Revision as of 12:43, 7 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

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VirA reporter system luc

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1195
Illegal NgoMIV site found at 2539
Illegal NgoMIV site found at 2560
Illegal AgeI site found at 2263
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2445

Design Notes

medium strong constitutive promoter
double terminator (forward) to keep basal expression of luciferase low
- double terminator BBa_B0017 instead of BBa_B0015 because of problems mentioned with BBa_B0012
luciferase gene to show the activity of the vir promoter

Source

virG gene synthesized by Mr. Gene (BBa_K389002)
vir promoter from A. tumefaciens C58 (BBa_K389003)
luciferase gene from Promega's pGL4.10luc2 vector (BBa_K389004)
constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)

References