Composite

Part:BBa_K389012:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-11)
Revision as of 14:59, 11 September 2010 by Registry (Talk | contribs)

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VirA reporter system luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2539
    Illegal NgoMIV site found at 2560
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2445


Design Notes

medium strong constitutive promotor, terminator to keep basal expression of luciferase low


Source

parts.igem, synthetic gene by Mr. Gene, pGL4.10luc2 by Promega, TI-plasmid from Agrobacterium tumefaciens C58

References