Composite
Part:BBa_K389015
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-09-28)
VirA/G reporter device luc
This BioBrick is for testing the virA/G signaling system. The read-out is the luciferase BBa_K389004. This system contains an unmutated virA, so it is possible to measure, how the natural virA/G system works and reacts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3769
Illegal NgoMIV site found at 5113
Illegal NgoMIV site found at 5134
Illegal AgeI site found at 4837 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768
Illegal SapI.rc site found at 5019
[edit]
Categories
Parameters
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//function/reporter/light
//chassis/prokaryote/ecoli
//function/reporter/light
None |