Device
"Lock"

Part:BBa_K318030:Design

Designed by: Justin Vrana, Nate Pantalone   Group: iGEM10_Wisconsin-Madison   (2010-07-13)
Revision as of 16:05, 27 August 2010 by N8pants (Talk | contribs) (→‎Design Notes)

lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
    Illegal NheI site found at 66
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 162


Design Notes

Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site after recombination.

Source

From Synthesis

References