Coding

Part:BBa_K385002:Design

Designed by: Krystal Annand, Joseph Hoare, Justyna Kucia, Stephen Lam   Group: iGEM10_Aberdeen_Scotland   (2010-09-22)
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Phage MS2 coat protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 331


Design Notes

We omitted the stop codon so this part could be used in a protein fusion construct, with the MS2 protein forming the N-terminal domain


Source

Phage MS2 genome (see NCBI sequence http://www.ncbi.nlm.nih.gov/nuccore/V00642.1)

References