Coding

Part:BBa_K322211

Designed by: William Rostain and Meng Lu   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 21:23, 25 September 2010 by Shenalu (Talk | contribs)

Red emitting firefly luciferase mutant (356K)

This is the same as BioBrick S284T but has lysine inserted between amino acids 355 and 356 of the native sequence. This has been reported to result in a red-shifted output.

Features: introduced lysine codon 1066 to 1068

reference:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


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