Coding

Part:BBa_J72037:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)
Revision as of 23:25, 15 July 2010 by Registry (Talk | contribs)

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{Bmll5} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR

Construction of MED7 Basic Part Bmll5

PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI)

Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)

Product is pBca9523-Bmll5

MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA

MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG

References