Coding

Part:BBa_I757011

Designed by: Freiburg iGEM 2007 team   Group: iGEM07_Freiburg   (2007-10-24)
Revision as of 08:31, 13 July 2010 by Kristian (Talk | contribs)

bla_frag1(aa 26-199) Fusion Part

  • first fragment of the split enzyme TEM beta-lactamase
  • purpose: split lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains
  • works with second half of split lactamase (part BBa_I757012)
  • this fragment contains stabilizing mutations
  • NgoMIV / AgeI protein fusion part
  • iGEM Team Freiburg 2007
  • Synthetic DNA by GeneArt
  • Part in pGA4 vector (AmpR, ColEI ori)
  • Part contains amino acids 26-199 of TEM-116 lactamase according to the numbering of Ambler, or amino acids 24-197 according to consecutive numbering with signal sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None