Generator
gmR

Part:BBa_P1014

Designed by: Tom Knight   Group: Part Editors   (2006-06-11)
Revision as of 14:35, 2 July 2010 by Tk (Talk | contribs)

gentamicin resistance cassette aph(2)

Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155

Conveys resistance to Genamicin at up to 25 ug/ml. It conveys low levels of resistance to Kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to Gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 5 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard Kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.

Gentamicin is an important antibiotic for use in human disease. Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/mflorum
//direction/forward
//plasmidbackbone/component/selectionmarker/antibioticresistance
Parameters
resistanceGentamicin 25 ug/ml