Coding
PS

Part:BBa_K343003:Design

Designed by: Christian Kurtzhals   Group: iGEM10_SDU-Denmark   (2010-07-02)
Revision as of 16:09, 2 July 2010 by Lclund (Talk | contribs) (→‎Design Notes)

NpSopII-NpHtrII-StTar (M-fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
    Illegal AgeI site found at 1585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal BsaI.rc site found at 1300
    Illegal SapI site found at 801
    Illegal SapI.rc site found at 1801


Design Notes

NpSopII-NpHtrII-EcTSR fusion-chimera protein sequence from Jung K-H, Spudich EN, Trivedi VD and Spudich JL; An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli; Journal of Bacteriology, Nov. 2001, p. 6365–6371. [http://jb.asm.org/cgi/content/full/183/21/6365]

Source

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References