Other
Part:BBa_J70500
Designed by: Tom Knight Group: Knight Lab (2009-09-13)
pWV01 broad host range plasmid origin and rep protein
pWV01 broad host range plasmid origin and rep protein. pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.
For use with mycoplasma species, the orfD seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/mflorum
None |