Cell

Part:BBa_V1005

Designed by: Tom Knight   Group: Arkin Lab   (2004-07-14)
Revision as of 15:08, 11 July 2006 by Smelissali (Talk | contribs)

DB3.1

E. coli strain containing the gyrA462 mutation, conveying recessive resistance to the ccdB gene.
Strain created by and available for purchase through Invitrogen


Usage and Biology

  • useful for propagating plasmids containing the ccdB operon.
  • gyrA462 enables ccdB containing plasmid propagation
  • streptomycin resistant


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeF- gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) supE44 ara14 galK2 lacY1proA2 rpsL20(Smr) xyl5 Δleu mtl1