Translational_Unit

Part:BBa_J18939

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)
Revision as of 19:32, 26 January 2010 by Registry (Talk | contribs)

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FKBP ~ bla-frag2

P01 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:

  • T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop

Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.

Expression

Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.

Reference

  • Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 589
    Illegal BsaI.rc site found at 20


[edit]
Categories
//cds
//proteindomain/binding
Parameters
None