Primer

Part:BBa_J18910

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-25)
Revision as of 09:38, 25 January 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RFC 25 vector forward (suffix)

Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 prefix but pointing outward into the backbone rather than insert.

Annealing temperature: 68 C (in Phusion polymerase buffer)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 11
    Illegal AgeI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None