Coding

Part:BBa_K5249029

Designed by: Jiasong Huang   Group: iGEM24_HBUT-China   (2024-10-01)
Revision as of 19:52, 1 October 2024 by Registry (Talk | contribs)

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122IsPETase

ISPETase is an enzyme that degrades PET and is produced by the bacterium Ideonella sakaiensis. The serine in it carries out nucleophilic attack, hydrolyzes the ester bonds in it, and produces TPA and EG from PET, and the intermediate products are MHET and BHET. We are also predicting its changing sites through machine learning and molecular docking, and expanding its substrate specificity and catalytic efficiency through structural changes

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
    Illegal NotI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 555
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 789
    Illegal PstI site found at 452
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None