Part:BBa_K5443014

Enoyl-CoA Hydratase/Aldolase (ECH) catalyzes the fifth and final step in the vanillin biosynthesis pathway, converting feruloyl-CoA to vanillin, thus completing the biosynthetic pathway. The enzyme belongs to the enoyl-CoA hydratase/isomerase superfamily and performs a two-step reaction, hydrating the double bond of feruloyl-CoA, then cleavage of the resulting thioester via a retro-aldol reaction. The ECH enzyme can also potentially use caffeoyl-CoA and 4-coumaroyl-CoA as substrates. We tested two FCS enzymes for this pathway step; this enzyme from Streptomyces, and also a homolog from Pseudomonas.

This part builds on the work of earlier iGEM teams, who contributed ECH-Str Parts as follows: Part:BBa_K2255000