DNA
PcW

Part:BBa_K5291022

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-11)
Revision as of 14:28, 30 September 2024 by Kortybones (Talk | contribs) (→‎Usage and Biology)


PcW

A common weak constitutive promoter in Pseudomonas aeruginosa.

Usage and Biology

We select PcW as the replacing promoter of hok/mok through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of sok is stronger than hok/mok when there is citrate in the environment, preventing cell death in advanced.


Fig.1 The schema of hok/sok system. The promoter of hok/mok is PcW.

We produce the hok/sok system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None