Coding

Part:BBa_K5322025:Design

Designed by: Xinyi Yang Yang   Group: iGEM24_NJTech-China   (2024-10-02)
Revision as of 12:40, 2 October 2024 by Casey (Talk | contribs)


SOD Plus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.


Source

The team obtains the SOD-1 sequence from BBa_K2215003. ===References=== 1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA. 2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(),49-60.https://doi.org/10.1016/j.ymben.2022.09.001 3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11