Coding
Part:BBa_K5051000:Design
Designed by: Yuk Ho Tsang Group: iGEM24_PLKNPL-HK (2024-10-01)
PKS1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1012
Illegal BglII site found at 1504
Illegal BglII site found at 4192
Illegal BglII site found at 5371
Illegal BglII site found at 5677 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2939
Illegal SapI.rc site found at 5579
Design Notes
It was codon optimized for Saccharomyces cerevisiae
Source
The PKS1 gene is from Aureobasidium pullulans EXF-150 genomic sequence