Composite

Part:BBa_K5236021

Designed by: Hening Guo, Yangzihan Chu   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 06:28, 2 October 2024 by Diarmuid (Talk | contribs)


pET28a-pelB-BhrPETase(N191S)-6xHis

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

BhrPETase N191S: This sequence encodes for a mutated BhrPETase N191S and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 116


[edit]
Categories
Parameters
None