Composite

Part:BBa_K5198107

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)
Revision as of 18:57, 23 August 2024 by Registry (Talk | contribs)

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nSEAP-NanoLuc

NanoLuciferase with native SEAP tag. This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901
    Illegal BsaI.rc site found at 1390


[edit]
Categories
Parameters
None