Device

Part:BBa_K188151

Designed by: shau-yi wu   Group: iGEM09_NCTU_Formosa   (2009-10-19)
Revision as of 18:50, 31 October 2009 by Poiuytrechu (Talk | contribs)

constitutive promoter +RBS+LacI+RBS+LuxR

Constitutive promoter (BBa_J23106) always express the downstream genes, LacI and LuxR (BBa_C0062). LacI inhibits the activity of the promoter PLac. If there is AHL surrounding, LuxR and AHL will form the complex and induce the expression of the promoter PcIIp22 (BBa_K145150).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aconstitutive promoter +RBS+LacI+RBS+LuxR