DNA
Part:BBa_K5443003:Experience
Designed by: Nick Coleman, Carmen Hawthorne Group: iGEM24_MacquarieUni (2024-09-27)
Revision as of 08:45, 2 October 2024 by Nickcoleman (Talk | contribs)
Applications of BBa_K5443003
We showed that the HpaBC enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of caffeate (see LC-MS data from one clone pMQ3C-11). Further evidence for functional expression of the HpaBC monooxygenase was seen from production of a brown melanin-type dye as a side-reaction; we believe this indicates that HpaBC is acting on tyrosine (to make L-DOPA, which polymerises) in addition to our intended reaction on 4-coumarate to make caffeate.
User Reviews
UNIQ4d31955c4d36f240-partinfo-00000000-QINU UNIQ4d31955c4d36f240-partinfo-00000001-QINU