Composite
PEBP-GFP

Part:BBa_K5291036

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 09:29, 2 October 2024 by Kortybones (Talk | contribs)


pAB1-pS-PEBP-GFP

This part is design to anchor the PEBP-GFP fusion protein to the membrane of bacteria. bacteria with PE-binding peptide (PEBP) are expected to have a stronger PE plastic binding capacity. And GFP is used to observe the bacteria on the PE surface to detect the binding ability of the engineering bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NotI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1033
    Illegal BamHI site found at 996
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1033
    Illegal PstI site found at 110
    Illegal PstI site found at 1043
    Illegal NgoMIV site found at 1351
    Illegal NgoMIV site found at 1720
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None