Composite

Part:BBa_K5127027

Designed by: Chujing Wu   Group: iGEM24_BNDS-China   (2024-10-02)
Revision as of 07:59, 2 October 2024 by Registry (Talk | contribs)

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IPTG-inducible promoter with IAA degradation enzyme (T7-iadCDE）

This composite part combines the T7 promotor and transcriptional unit iadCDE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2191
    Illegal PstI site found at 2769
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2374
    Illegal NheI site found at 696
    Illegal PstI site found at 2191
    Illegal PstI site found at 2769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2374
    Illegal BglII site found at 2383
    Illegal BglII site found at 2435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2191
    Illegal PstI site found at 2769
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2191
    Illegal PstI site found at 2769
    Illegal NgoMIV site found at 642
    Illegal NgoMIV site found at 1633
    Illegal AgeI site found at 406
    Illegal AgeI site found at 1435
    Illegal AgeI site found at 1876
    Illegal AgeI site found at 2299
    Illegal AgeI site found at 2733
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None