Part:BBa_K5115001

hoxF, without stop codon

Introduction

The hoxF is a subunit of the hox and hyp operon BBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115011(ribozyme+RBS+hoxF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 577
  Illegal BglII site found at 1395
  Illegal BglII site found at 1688
  Illegal XhoI site found at 22
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 456
  Illegal NgoMIV site found at 994
  Illegal NgoMIV site found at 1204
  Illegal NgoMIV site found at 1516
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 625
  Illegal BsaI.rc site found at 1129