Composite
Delay test

Part:BBa_K257021:Experience

Designed by: Romain Bodinier   Group: iGEM09_Paris   (2009-10-19)
Revision as of 04:01, 22 October 2009 by Benoît dH (Talk | contribs) (→‎Applications of BBa_K257021)

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Applications of BBa_K257021


PtetRFP trans.jpg
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction


PtetRFP fluo.jpg
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction


Ptet RFP Glucose trans1.jpg
x1000 observation on white light of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose


Ptet RFP Glucose fluo1.jpg
x1000 observation after fluorescence excitation of E. coli with the pLac-TetR::pTet-mRFP construction in presence of 1% glucose


Conclusion :
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction more bacteria are fluorescent in presence of glucose than in the control.

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