Plasmid

Part:BBa_K197038

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-25)
Revision as of 18:43, 25 October 2009 by Registry (Talk | contribs)

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BBb - BseRI/AcuI entry vector

This is an entry vector used for housing basic parts before entry into 2AB layered assembly (an assembly scheme developed at UC Berkeley).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2472
    Illegal XhoI site found at 1486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
    Illegal NgoMIV site found at 1339
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
origincoE1
resistanceS