Coding

Part:BBa_K5035008:Design

Designed by: Lucas Garcia   Group: iGEM24_IEA   (2024-10-02)
Revision as of 04:17, 2 October 2024 by Registry (Talk | contribs)

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AI_1 Generated Reductive Dehalogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 1317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 436


Design Notes

AI_1 was given a hexahistidine tag to allow for nickel column protein purification.


Source

https://www.biorxiv.org/content/10.1101/2024.07.01.600583v1

References