Coding
LovTAP

Part:BBa_K191006

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-16)
Revision as of 13:03, 21 October 2009 by Nltt1987 (Talk | contribs)

LOVTAP

Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535


[edit]
Categories
//function/sensor/light
Parameters
None