Regulatory

Part:BBa_K239006:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-26)
Revision as of 16:33, 26 June 2009 by Registry (Talk | contribs)

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Modified NarK promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.


Source

Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.


References