Part:BBa_K5115066

mineral, U module

Introduction

Most of the U module is the same with BBa_K5115067（mineral, F module）, except for that in U module the EP is combined with hoxU and in F module the EP is combined with F module. We design these two parts to compare out which subunit for EP to combine with is more effective. The final experiment shows that module F works better than module U. For more details about the module and experiment, please check BBa_K5115067（mineral, F module）.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 208
  Illegal NotI site found at 5126
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 366
  Illegal BglII site found at 5912
  Illegal BglII site found at 6730
  Illegal BglII site found at 7023
  Illegal BglII site found at 15401
  Illegal BglII site found at 15479
  Illegal BamHI site found at 7317
  Illegal XhoI site found at 5357
  Illegal XhoI site found at 7524
  Illegal XhoI site found at 9763
  Illegal XhoI site found at 11592
  Illegal XhoI site found at 12754
  Illegal XhoI site found at 14593
  Illegal XhoI site found at 15409
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 5791
  Illegal NgoMIV site found at 6329
  Illegal NgoMIV site found at 6539
  Illegal NgoMIV site found at 6851
  Illegal NgoMIV site found at 7617
  Illegal NgoMIV site found at 8297
  Illegal NgoMIV site found at 10904
  Illegal AgeI site found at 874
  Illegal AgeI site found at 1825
  Illegal AgeI site found at 2506
  Illegal AgeI site found at 3460
  Illegal AgeI site found at 12079
  Illegal AgeI site found at 15362
  Illegal AgeI site found at 16361
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 9417
  Illegal BsaI site found at 15183
  Illegal BsaI site found at 15345
  Illegal BsaI.rc site found at 5960
  Illegal BsaI.rc site found at 6464
  Illegal SapI site found at 266
  Illegal SapI.rc site found at 15294