Part:BBa_K5233023:Design

TurboPETase(pelB), a designed enzyme that can be secreted into the extracellular space

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 78
  Illegal AgeI site found at 223
  Illegal AgeI site found at 579
  Illegal AgeI site found at 624
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Because PET is extracellular, we need to secrete TurboPETase into the extracellular space with a signal peptide. Enhancers can enhance signal peptide effects.

Source

The TurboPETase comes from an assay called "Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading". The pelB comes from pectate lyase B from Pectobacterium carotovorum. The enhancer comes from an assay called "Excretory expression of IsPETase in E. coli by an enhancer of signal peptides and enhanced PET hydrolysis".

References

Cui Y, Chen Y, Sun J, Zhu T, Pang H, Li C, Geng WC, Wu B. Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading. Nat Commun. 2024 Feb 15;15(1):1417. doi: 10.1038/s41467-024-45662-9. PMID: 38360963; PMCID: PMC10869840.