Part:BBa_K5291003
vgb: Vitreoscilla hemoglobin
vgb encodes VHb. VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to improve the degradation ability of CYPY96F on PE.
Usage and Biology
In degradation module we constructed pAB1-CYPY96F-VHb to ensure the expression of emzymes related to degradation.
Fig.1 The map of plasmid pAB1-CYPY96F-VHb.
We transferred the constructed plasmid into Escherichia coli DH5αstrain, conducted colony PCR and obtained the correct result.
Fig.2 P.aeryginosa PAO1 colony PCR results of CYPY96F-VHB.
Moreover,VHb proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.
Fig.3 The SDS-PAGE result of VHb.
Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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