Part:BBa_K5185032
rtUlp1（Recombinant truncated Ubiquitin-like protease 1）
Small Ubiquitin-like Modifier (SUMO) is a ubiquitin-related protein that regulates the function of target proteins by binding to lysine residues. It can be recognized and cleaved by SUMO proteases, leaving no amino acid residues upon cleavage by SUMO tag proteases. This can increase the solubility of expressed proteins, affect the folding of C-terminal fusion proteins, and protect target peptides from degradation by proteases, making it commonly used for expressing small antimicrobial peptides. Although Ulp1 is an attractive tool for obtaining target proteins with natural N-terminal sequences, its application in cutting SUMO fusion proteins is greatly restricted due to its expensive price and complex procedures. rtUlp retains the Leu1403-Lys631 of yeast Ulp, making it smaller in molecular weight and easier to recombinantly express.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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