Coding
Part:BBa_K197014
Designed by: Gabriela Guzman Lopez Aguado Group: iGEM09_Berkeley_Wetlab (2009-10-21)
{AIDA-I}
Features: identified to be similar to IgA1, the first autotransporter used for surface display. Occurs naturally in the host organism, E. coli, and is a robust tool for surface display. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 56
Illegal PstI site found at 339 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 56
Illegal PstI site found at 339 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 56
Illegal PstI site found at 339 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 56
Illegal PstI site found at 339
Illegal AgeI site found at 771 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |