Coding

Part:BBa_K197014

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 03:48, 22 October 2009 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


{AIDA-I}

Features: identified to be similar to IgA1, the first autotransporter used for surface display. Occurs naturally in the host organism, E. coli, and is a robust tool for surface display. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
    Illegal AgeI site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None