Composite

Part:BBa_K5106010

Designed by: Nishant Kulkarni   Group: iGEM24_WageningenUR   (2024-10-01)
Revision as of 20:49, 1 October 2024 by Registry (Talk | contribs)

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Toehold AND gate, T7 promoter and terminator, LacZ reporter

MS toehold A (BBa_K5106003) under control of a T7 promoter and terminator, regulating expression of a LacZ reporter gene, in order to test the toehold functionality in vitro in a cell-free PURExpress system.

Basic toehold AND gate (BBa_K5106007 under a T7 promoter and terminator, regulating LacZ reporter gene, to test the basic construct in vitro in a cell-free PURExpress system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None