Part:BBa_K5101007:Design
Certolizumab (Anti-TNF-α )Heavy Chain With OmpA
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 603
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 603
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 603
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 603
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 603
Illegal AgeI site found at 585 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The genes encoding these antibody fragments were synthesized based on sequences adapted for bacterial expression and optimized for E. coli, including an ompA signal sequence to direct the proteins to the periplasm. This localization is critical for proper protein folding and disulfide bond formation, essential for the functionality of the antibody. Certolizumab Pegol, used as the model for these fragments, is known for its efficacy in neutralizing TNF-α without Fc-mediated functions, which can be advantageous in reducing immunogenicity. For validation and scientific comparison, similar sequences used in this design are accessible in the NCBI database, which provides extensive data on various therapeutic antibodies.
Source
The genes encoding these antibody fragments were synthesized based on sequences adapted for bacterial expression and optimized for E. coli, including an ompA signal sequence to direct the proteins to the periplasm. This localization is critical for proper protein folding and disulfide bond formation, essential for the functionality of the antibody. Certolizumab Pegol, used as the model for these fragments, is known for its efficacy in neutralizing TNF-α without Fc-mediated functions, which can be advantageous in reducing immunogenicity. For validation and scientific comparison, similar sequences used in this design are accessible in the NCBI database, which provides extensive data on various therapeutic antibodies.