Composite

Part:BBa_K197034

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 03:57, 22 October 2009 by Registry (Talk | contribs)

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{Pbad.rbs.prepro.StrepTag}{mgfp-5}{GFP-LVA}{cl02365 AtD}(dblterm)

This is a cell surface display device that exposes mgfp-5 (a mussel foot adhesion protein) to the extracellular environment via fusion to the putative autotransporter {cl02365 AtD}. When expressed in the correct environment mgfp-5 should form an underwater stable bio-glue. This device will enable engineered E.coli to bind to surfaces.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
    Illegal PstI site found at 2412
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2412
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2337
    Illegal SapI site found at 1005


[edit]
Categories
Parameters
None